SAB4200379

Anti-JMY antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 同義詞: Anti-Junction mediating and regulatory protein, Anti-p53 cofactor
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~120 kDa
• 物種活性: human
• 濃度: ~1.0 mg/mL
• 技術: immunoprecipitation (IP): 5-10 μg using lysates of human WiDr cells.; indirect immunofluorescence: 1-2 μg/mL using human HeLa cells.; western blot: 0.5-1.0 μg/mL using whole extracts of human WiDr cells
• UniProt登錄號: Q8N9B5
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... JMY(133746)

描述

一般說明

Junction mediating and regulatory protein (JMY) is identified as a transcriptional co-activator of p53 and is a multifunctional actin nucleation factor. JMY contains Wiskott-Aldrich syndrome protein (WASp)-homology 2 (WH2) domains in its C-terminus. The JMY gene is mapped to human chromosome location 5q14.1.

特異性

Anti-JMY recognizes human JMY.

免疫原

synthetic peptide corresponding to the C-terminal region of human JMY, conjugated to KLH.

應用

Anti-JMY antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence.

生化／生理作用

Junction mediating and regulatory protein (JMY) acts as a nucleation promoting factor by activating actin-related proteins-2/3 (ARP2/3), creating branched filament networks, and also by assembling unbranched filaments directly through a Spire-like mechanism. Overexpression of JMY increases motility while its loss slows cell migration. DNA damage causes JMY to accumulate in the nucleus, where it enhances p53-dependent transcription of pro-apoptotic genes. The activities of JMY as a p53 co-factor or as an actin nucleation factor are regulated by its subcellular localization.

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable