生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
IgG fraction of antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~12 kDa
物種活性
mouse, rat, human
技術
immunohistochemistry: 1:100-1:200 using formalin-fixed paraffin-embedded human breast carcinoma
indirect immunofluorescence: 1:250-1:500 using HeLa cells
western blot: 1:500-1:1000 using NIH3T3 cell lysates
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
acetylation (Lys12)
基因資訊
human ... HIST3H3(8290)
一般說明
Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. It is an important component of chromatin structure.
免疫原
synthetic peptide containing acetylated Lys12 of human histone H4, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H4.
應用
Anti-acetyl-Histone H4 [Ac-Lys12] antibody produced in rabbit has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
生化／生理作用
Histone H4 can be reversibly acetylated at lysine residues K5, K8, K12 and K16, and the acetylation occurs initially at K16 and then propagates in an N-terminal direction through K12, K8 and K5. Altered acetylation of histone H4 on Lys12 (H4- K12Ac) is linked to age-dependent memory impairment in mice. Histones are subjected to several covalent modifications, such as phosphorylation, methylation, acetylation and ubiquitination, that affect chromatin structure and regulate chromatin activity. Histone modifications plays an important role in cancer and disease.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Altered histone acetylation is associated with age-dependent memory impairment in mice
Peleg S, et al.
Science, 328(5979), 753-756 (2010)
Robert Schneider et al.
Trends in biochemical sciences, 27(8), 396-402 (2002-08-02)
Enzymes that covalently modify histones control many cellular processes by affecting gene expression. A new class of these enzymes is the histone lysine methyltransferase family, whose catalytic activity lies within a conserved domain, the SET domain. This article surveys the
The language of covalent histone modifications
Strahl BD and Allis CD
Nature, 403(6765), 41-41 (2000)
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