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SAB4200190

Sigma-Aldrich

Anti-Sumo-2/3 antibody, Rat monoclonal

clone 3H12, purified from hybridoma cell culture

同義詞:

Anti-HSMT3, Anti-MGC117191, Anti-SMT3 homolog 2, Anti-SMT3 suppressor of mif two 3 homolog 2, Anti-SMT3B, Anti-SMT3H2, Anti-sentrin 2, Anti-small ubiquitin-like modifier 2, Anti-small ubiquitin-related modifier 2, Anti-ubiquitin-like protein SMT3B, Monoclonal Anti-Sumo-2/3, SUMO-2/3, SUMO2/3

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

共軛

unconjugated

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

3H12, monoclonal

形狀

buffered aqueous solution

分子量

~11 kDa

物種活性

mouse, rat, human

濃度

~1.0 mg/mL

技術

immunocytochemistry: suitable
western blot: 2-4 μg/mL using HeLa cell nuclear extract

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... SUMO3(6612)
mouse ... Sumo3(20610)
rat ... Sumo3(499417)

相關類別

一般說明

Monoclonal Anti-Sumo-2/3 (rat IgG2a isotype) is derived from the hybridoma 3H12 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a human SUMO3 fusion protein.
Small ubiquitin-like modifier 3 (SUMO3) is encoded by the gene mapped to human chromosome 21q22.3. The protein belongs to the ubiquitin-like protein family and is expressed mainly in cytoplasm.

免疫原

human SUMO3 fusion protein

應用

Monoclonal Anti-Sumo-2/3 has been used in immunoblotting and immunocytochemistry.

生化/生理作用

Small ubiquitin-like modifier (SUMO) was identified as a post-translational protein modifier.
Small ubiquitin-like modifier 3 (SUMO3) plays a vital role in enhancing cellular responses to environmental stress. It acts as a key element of the leukemogenic network in transient myeloproliferative disorder (TMD) of the newborn/acute megakaryoblastic leukaemia (AMKL).

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3.
Saitoh H and Hinchey J.
The Journal of Biological Chemistry, 275, 6252-6258 (2000)
SUMO-2/3 conjugates accumulating under heat shock or MG132 treatment result largely from new protein synthesis.
Castoralova M
Biochimica et Biophysica Acta, 1823, 911-919 (2012)
Molecular features of human ubiquitin-like SUMO genes and their encoded proteins
Su HL and Li SS
Gene, 296, 65-73 (2002)
The Small Ubiquitin-Like Modifier (SUMO) and SUMO-Conjugating System of Chlamydomonas reinhardtii
Wang Y, et al.
Genetics, 179(1), 177-177 (2008)
A 15q24 microdeletion in transient myeloproliferative disease (TMD) and acute megakaryoblastic leukaemia (AMKL) implicates PML and SUMO3 in the leukaemogenesis of TMD/AMKL
Haemmerling S
British Journal of Haematology, 157, 180-187 (2012)

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