生物源
rabbit
共軛
unconjugated
抗體表格
IgG fraction of antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen 75 kDa
物種活性
mouse, rat, human
包裝
antibody small pack of 25 μL
技術
immunoprecipitation (IP): suitable
western blot: 1:500-1:1,000 using HeLa cell lysates
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... RAD17(5884)
mouse ... Rad17(19356)
rat ... Rad17(310034)
一般說明
Cell cycle checkpoint protein RAD17 is mapped to human chromosome 5q13.2 . It is also called s a replication factor C (RFC)-like DNA damage sensor protein. Eight alternative spliced transcript variants of the gene, which encode four distinct proteins, have been reported.
免疫原
synthetic peptide corresponding to amino acids 2-15 of human RAD17 (isoform 1), conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide differs from that of isoform 2 by two amino acids.
應用
Anti-RAD17 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.
生化／生理作用
RAD17 has been reported in breast, non-small cell lung carcinoma and breast cancer. RAD17 is a critical event during checkpoint signaling in DNA-damaged cells. Treatment of human cells with genotoxic agents induced ATM/ATR dependent phosphorylation of RAD17 at Ser635 and Ser645 , suggesting that phosphorylation of RAD17 is a critical event during checkpoint signaling in DNA-damaged cells.
外觀
Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Human Rad17 is phosphorylated upon DNA damage and also overexpressed in primary non-small cell lung cancer tissues
Wang X, et al.
Cancer research, 61(20), 7417-7421 (2001)
Basal-like Breast cancer DNA copy number losses identify genes involved in genomic instability, response to therapy, and patient survival
Weigman VJ, et al.
Breast Cancer Research and Treatment, 133(3), 865-880 (2012)
Rad17 recruits the MRE11-RAD50-NBS1 complex to regulate the cellular response to DNA double-strand breaks
Wang Q, et al.
The Embo Journal, 33(8), 862-877 (2014)
Yasunori Fukumoto et al.
The Journal of biological chemistry, 297(2), 100831-100831 (2021-06-27)
The ATR pathway is one of the major DNA damage checkpoints, and Rad17 is a DNA-binding protein that is phosphorylated upon DNA damage by ATR kinase. Rad17 recruits the 9-1-1 complex that mediates the checkpoint activation, and proteasomal degradation of
Eun Kyeong Oh et al.
International journal of oncology, 41(6), 2038-2046 (2012-10-02)
Host genomic alterations in addition to human papillomavirus (HPV) are needed for cervical precursor lesions to progress to invasive cancer because only a small
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