QPREST27836

SILu^™PrEST ZCPW2

SILuPrESTs Powered by Atlas Antibodies, buffered aqueous solution

• 分類程式碼代碼: 12352200

屬性

• 重組細胞: expressed in E. coli LysA ArgA BL21(DE3)
• 化驗: >80% (SDS-PAGE)
• 形狀: buffered aqueous solution
• 分子量: predicted mol wt 26kDa including tags
• 純化經由: immobilized metal affinity chromatography (IMAC)
• 包裝: pkg of 1nmol × 5 vials
• 儲存條件: avoid repeated freeze/thaw cycles
• 免疫原序列: TATPDESEEGHGEEINMGEKLSKCSPEAPAGSLFENHYEEDYLVIDGIKLKAGECIEDITNKFKEIDALMSEF
• UniProt登錄號: Q504Y3
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... ZCPW2(152098)

描述

一般說明

Lys, Arg ¹³C and ¹⁵N metabolically labeled recombinant human protein fragment

應用

Internal standard in MS-based quantitative proteomics

外觀

Heavy proteins are provided in solution of 1M Urea PBS, pH 7.4

準備報告

The protein solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

分析報告

Isotopic Label Incorporation

The SILuPrEST standards are produced using metabolic labeling with heavy isotope labeled (15N, 13C) Lysine and Arginine residues, with greater than 99% isotopic label incorporation.

A purification and quantification tag (QTag) consisting of a hexahistidine (His6) sequence followed by an Albumin Binding Protein (ABP) domain derived from Streptococcal Protein G.

All SILuPrESTs contain an N-terminal His6ABP fusion tag, used for accurate determination of concentration. The fusion tag sequence is:

GSSHHHHHHSSGLVPRGSHMASLAEAKVLANRELDKYGVSDYHKNLINNAKTVEGVKDLQAQVVESAKKARISEATDGLSDFLKSQTPAEDTVKSIELAEAKVLANRELDKYGVSDYYKNLINNAKTVEGVKALIDEILAALPGTFAHYMDPNSSSVDKLAAA.

The specific human protein sequence begins directly after ′VDKLAAA′.

法律資訊

This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com

SILu is a trademark of Sigma-Aldrich Co. LLC

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable