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PP0520

Sigma-Aldrich

GlycoProfile™ 2-AB Labeling Kit

同義詞:

2-AB Labeling Kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

一般說明

The GlycoProfile™ labeling kit专门设计用于高效地使用2-氨基苯甲酰胺（2-AB）或2-氨基苯甲酸（氨茴酸；2-AA）来对N-连接、O-连接、及糖基磷脂酰肌醇（GPI）锚定的多糖进行高效标记。这些小荧光素可增强多糖的光谱吸收，提高HPAE（高性能阴离子交换色谱）、HPLC、及ESI-MS（电喷射质谱）等方法标记多糖的检测效果。其结合非常可靠，所得的染料-多糖轭合物也很稳定，在标记后的分析过程中不会发生降解。

應用

GlycoProfile™ 2-AB标记试剂盒用于标记人IgG、牛胎球蛋白和人附睾蛋白4中的N-连接聚糖。也用于标记小鼠脾脏中的游离寡糖。
虽然2-AB的灵敏度略低于2-AA，但其适用于HPAE、HPLC和ESI-MS进行下游聚糖分析。 尽管2-AA适用于相同应用，但其更适合用于SDS-PAGE。

法律資訊

使用2-AB进行多糖标记的方法已获得美国专利号5、747、347及海外相应专利。
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

僅套裝組件

產品號碼
描述
  • 2-AB Labeling Reagent 2 x 5
  • Dimethyl sulfoxide (DMSO) 2 x 350
  • Acetic acid, glacial 2 x 200
  • Reductant (sodium cyanoborohydride) 2 x 6

訊號詞

Danger

危險分類

Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Sol. 1 - Skin Corr. 1A - Water-react 1

安全危害

EUH032

儲存類別代碼

4.3 - Hazardous materials which set free flammable gases upon contact with water

分析證明 (COA)

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Separation of 2-aminobenzamide labeled glycans using hydrophilic interaction chromatography columns packed with 1.7 mum sorbent
Ahn J, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 878(3-4), 403-408 (2010)
Florian Cambay et al.
Current research in immunology, 1, 23-37 (2020-06-27)
The effector functions of the IgGs are modulated by the N-glycosylation of their Fc region. Particularly, the absence of core fucosylation is known to increase the affinity of IgG1s for the Fcγ receptor IIIa expressed by immune cells, in turn
Enhancing the antiviral potency of ER alpha-glucosidase inhibitor IHVR-19029 against hemorrhagic fever viruses in vitro and in vivo
Ma J, et al.
Antiviral research, 150, 112-122 (2018)
Expression and biochemical characterization of recombinant human epididymis protein 4
Hua L, et al.
Protein Expression and Purification, 102, 52-62 (2014)
Céline Raymond et al.
mAbs, 7(3), 571-583 (2015-04-16)
The presence of α2,6-sialic acids on the Fc N-glycan provides anti-inflammatory properties to the IgGs through a mechanism that remains unclear. Fc-sialylated IgGs are rare in humans as well as in industrial host cell lines such as Chinese hamster ovary
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