P8996

Monoclonal Anti-PINCH-1 antibody produced in mouse

clone PINCH-C58, purified immunoglobulin, buffered aqueous solution

• MDL號碼: MFCD06798713
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: PINCH-C58, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~37 kDa
• 物種活性: hamster, bovine, mouse, monkey, canine, human, rat
• 技術: immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using total cell extract from 293T cells
• 同型: IgG1
• UniProt登錄號: P48059
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... LIMS1(3987) ; mouse ... Lims1(110829) ; rat ... Gcc2(309798)

描述

一般說明

Monoclonal Anti-PINCH-1 (mouse IgG1 isotype) is derived from the hybridoma PINCH-C58 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a peptide of human PINCH1, conjugated to KLH. LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) or particularly interesting new Cys-His protein 1 (PINCH-1) is widely expressed. It has five LIM domains. It has leucine-rich nuclear export signal (NES) and nuclear localization signal (NLS) at the C-terminus. Human PINCH-1 and 2 are encoded by two different genes that are 82% identical in their amino acid sequence.

免疫原

peptide corresponding to amino acids 281-299 of human PINCH-1, conjugated to KLH.

應用

Monoclonal Anti-PINCH-1 antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• co-immunoprecipitation

生化／生理作用

The interaction with PINCH proteins (PINCH-1 and 2) is important for localization of integrin linked kinase protein to cell matrix contact sites. It plays a key role in cell−matrix adhesion. PINCH2 is co-expressed and co-localized with PINCH-1/ILK complex. PINCH1 interacts with Wilms tumor 1 protein (WT1) in podocytes. Elevated expression of PINCH1 is observed in human laryngeal carcinoma.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable