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Merck
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重要文件

P8248

Sigma-Aldrich

Monoclonal Anti-Profilin antibody produced in mouse

~2 mg/mL, clone 2B8 (MAbPRF45a), purified immunoglobulin, buffered aqueous solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352203

生物源

mouse

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

2B8 (MAbPRF45a), monoclonal

形狀

buffered aqueous solution

分子量

antigen ~14 kDa

物種活性

Arabidopsis thaliana

濃度

~2 mg/mL

技術

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using total extracts of Arabidopsis flower

同型

IgG1

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

Arabidopsis thaliana ... PRF4(829055)

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一般說明

Profilins are highly conserved pan-allergens that occur in plant foods, pollen and latex. Profilins have five distinct isoforms (PRF1-5) that bind with actin filaments to regulate cytoskeletal organization. PRF4 and PRF5 are cytoplasmic proteins that are expressed in mature pollens . Monoclonal Anti-Profilin antibody is specific for Arabidopsis pollen profilin (PRF4 and PRF5). The antibody detects tobacco and maize profilin (approx. 14 kDa), but does not react with constitutive specific profilins (PRF1, 2, and 3).

免疫原

profilin PRF4 protein.

應用

Monoclonal Anti-Profilin antibody is suitable for use in western blot (1-2 μg/mL using total extracts of Arabidopsis flower), indirect ELISA and immunohistochemistry.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Muthugapatti K Kandasamy et al.
Cell motility and the cytoskeleton, 52(1), 22-32 (2002-04-27)
Profilin is a low-molecular weight, actin monomer-binding protein that regulates the organization of actin cytoskeleton in eukaryotes, including higher plants. Unlike the simple human or yeast systems, the model plant Arabidopsis has an ancient and highly divergent multi-gene family encoding
Alexandra Santos et al.
International archives of allergy and immunology, 155(3), 191-204 (2011-02-05)
Profilins are ubiquitous proteins, present in all eukaryotic cells and identified as allergens in pollen, latex and plant foods. The highly conserved structure justifies the cross-reactive nature of IgE antibodies against plant profilins and their designation as pan-allergens. Primary sensitization

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