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P1253

Sigma-Aldrich

卵黄高磷蛋白 来源于鸡蛋黄

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.81

生物源

egg yolk

形狀

powder

儲存溫度

−20°C

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一般說明

蛋黄中的卵黄高磷蛋白是富含聚阴离子磷酸丝氨酸的蛋白质。该磷酸糖蛋白包含α-螺旋和β-折叠结构元件,由于磷酸丝氨酸的存在,它们的组织不良。它代表约11%的卵黄蛋白,并且是亲水性的。

應用

蛋黄中的卵黄高磷蛋白已用于:
  • 作为对照蛋白固定在芯片上用于深紫色蛋白染色,用于监测翻译后修饰
  • 作为体外酪蛋白激酶测定中酪蛋白激酶Iε的底物
  • 作为凝胶过滤色谱(GFC)和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)中的标准品,用于定量测定蛋黄提取物中的卵黄高磷蛋白
  • 用于逐层(LbL)固定化,用于傅里叶变换红外光谱衰减全反射(FTIR-ATR)研究

生化/生理作用

蛋黄中的卵黄高磷蛋白是一种钙螯合剂,可与蛋黄中存在的铁结合。金属结合功能被用于矿物结合生物活性肽的生产中。它是一种营养保健品,具有磷酸肽生产的潜力。
通过在食物中添加蛋黄喂养大鼠的卵黄高磷蛋白导致镁、铁和钙的吸收减少,但磷的吸收没有减少。

其他說明

含8-10%磷的磷蛋白。 摩尔N/P比约为2.7。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Samooel Jung et al.
Food chemistry, 135(3), 993-998 (2012-09-08)
Phosvitin is a phosphoglycoprotein present in egg yolk. More than half of the amino acids in phosvitin molecule are serine, of which >90% are phosphorylated. Therefore, phosvitin has a strong metal binding capability. The aim of this study was to
Shuichi Ito et al.
Journal of biomedical materials research. Part A, 100(10), 2760-2765 (2012-05-25)
The aim of this study is to evaluate the mineralizing potential of acidic monomers and their calcium salts for mineralization, using an in vitro mineral induction model. Phosvitin (PV) was used as a model phosphoprotein in this study. PV was
K Y Ko et al.
Poultry science, 90(5), 1096-1104 (2011-04-15)
The objective of this study was to develop a new protocol that could be used for large-scale separation of phosvitin from egg yolk using ethanol and salts. Yolk granules, which contain phosvitin, were precipitated after diluting egg yolk with 9
Shuichi Ito et al.
Journal of dentistry, 39(1), 72-79 (2010-10-26)
The aim of this study was to evaluate the mineralizing potential of ions released from surface pre-reacted glass-ionomer (S-PRG) fillers on mineral induction by phosphoprotein in vitro. Phosvitin was used as a model of dentin phosphoprotein in this study. Phosvitin
S-I Ishikawa et al.
Journal of food science, 72(6), S412-S419 (2007-11-13)
Egg yolk decreases the absorption of iron. The effects of egg yolk protein and egg yolk phosvitin on the absorption of calcium, magnesium, and iron were investigated by in vivo studies. Male Wistar rats were fed purified diets containing casein

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