跳轉至內容
Merck
全部照片(1)

重要文件

NUC101

Sigma-Aldrich

细胞核分离试剂盒:Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

同義詞:

Rapid mammalian nuclei isolation kit

登入查看組織和合約定價


About This Item

分類程式碼代碼:
12352207
NACRES:
NA.32

用途

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

品質等級

儲存期限

1 yr at 2‑8 °C

包裝

pkg of 1 kit

應用

cell analysis

異物活動

nuclease and protease, free

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

Nuclei EZ Prep Kit用于从哺乳动物细胞中快速分离胞核。该试剂盒可从通用细胞类型获得高得率细胞核。

應用

适于作为细胞核成分的来源,为体外凋亡检测和功能研究提供细胞核成分。

生化/生理作用

配套实验方案使您可从常用的哺乳动物细胞获得高得率的细胞核,包括贴壁 (例如,HEK293和COS7)和非贴壁(例如,Jurkat和HFN7.1)细胞系和外周血单核细胞(PBMC)。该制备适用于许多细胞生物学应用,例如:为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源,为体外凋亡检测、功能性研究(如细胞转录状态检查)制备细胞核。

其他說明

所有操作步骤应在冰块上或2-8°C条件下进行。

相關產品

產品號碼
描述
訂價

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Srikanth Pendyala et al.
Antioxidants & redox signaling, 11(4), 747-764 (2008-09-12)
In vascular endothelium, the major research focus has been on reactive oxygen species (ROS) derived from Nox2. The role of Nox4 in endothelial signal transduction, ROS production, and cytoskeletal reorganization is not well defined. In this study, we show that
Baochen Shi et al.
BMC genomics, 10, 92-92 (2009-02-27)
A major goal of post-genomics research is the integrated analysis of genes, regulatory elements and the chromatin architecture on a genome-wide scale. Mapping DNase I hypersensitive sites within the nuclear chromatin is a powerful and well-established method of identifying regulatory
Identification of newly transcribed RNA.
Greenberg, M.E., and Bender, T.P., et al. et al.
Current Protocols in Molecular Biology, 4-4 (1987)
Bas Castelijns et al.
Nature communications, 11(1), 301-301 (2020-01-18)
Speciation is associated with substantial rewiring of the regulatory circuitry underlying the expression of genes. Determining which changes are relevant and underlie the emergence of the human brain or its unique susceptibility to neural disease has been challenging. Here we

文章

亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務