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NDEGLY

Sigma-Aldrich

天然蛋白去糖基化试剂盒

同義詞:

Protein Deglycosylation Kit

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

共軛

(N-linked)

品質等級

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

天然蛋白去糖基化设计用于 PNGase F 抗性天然蛋白 N-连接寡糖的去糖基化。内切糖苷酶 F1、F2 和 F3 对蛋白质构象的敏感性低于 PNGase F,更适用于去除所有类别的 N-连接寡糖而不使蛋白质变性。
用神经氨酸酶、 β-半乳糖苷酶和 N-乙酰氨基葡萄糖苷酶处理糖蛋白,可将所有复杂的寡糖还原为三甘露糖基壳二糖核心。在某些情况下可能需要岩藻糖苷酶。残留的三甘露糖基壳二糖核心结构则可通过内切糖苷酶F3去除。内切糖苷酶F2和F3可即刻分别去除双触角和三触角结构。内切糖苷酶F1可去除低聚甘露糖和杂合结构。
每种类型内切糖苷酶的更多相关信息,请参阅公告。

應用

天然蛋白去糖基化试剂盒用于各种酶的 N-去糖基化,适用漆酶、 番茄核酸酶TBN1和内切-β-1,3-葡聚糖酶。

儲存和穩定性

NDEGLY 套件用湿冰运输,建议储存在2°C至8°C条件下。如按照指示说明储存,则该套件有效期至少为一年。只在环境温度下暴露几天,不会降低该试剂盒中酶的活性。

僅套裝組件

產品號碼
描述

  • Endoglycosidase F1 .3 U

  • Endoglycosidase F2 .1 U

  • Endoglycosidase F3 .1 U

  • Endoglycosidase F1 reaction buffer 200 μL

  • Endoglycosidase F2 & 3 reaction buffer 200 μL

儲存類別代碼

12 - Non Combustible Liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Junyi Ma et al.
Molecular pharmacology, 69(4), 1137-1145 (2006-01-10)
Opioid analgesics remain the choice for the treatment of moderate to severe pain. Recent research has established that the mu-opioid receptor is predominantly responsible for mediating many opioid actions, including analgesia and opioid tolerance. However, the function of delta-opioid receptors
Adela Rodríguez-Romero et al.
Acta crystallographica. Section D, Biological crystallography, 70(Pt 2), 329-341 (2014-02-18)
Endogenous glycosylated Hev b 2 (endo-β-1,3-glucanase) from Hevea brasiliensis is an important latex allergen that is recognized by IgE antibodies from patients who suffer from latex allergy. The carbohydrate moieties of Hev b 2 constitute a potentially important IgE-binding epitope
N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity
Podzimek T, et al.
Plant Science (2018)
Amir E Zeituni et al.
Journal of bacteriology, 192(16), 4103-4110 (2010-06-22)
We recently reported that the oral mucosal pathogen Porphyromonas gingivalis, through its 67-kDa Mfa1 (minor) fimbria, targets the C-type lectin receptor DC-SIGN for invasion and persistence within human monocyte-derived dendritic cells (DCs). The DCs respond by inducing an immunosuppressive and
Laccase isoform diversity in basidiomycete Lentinus strigosus 1566: Potential for phenylpropanoid polymerization
Kolomytseva MP, et al.
International Journal of Biological Macromolecules (2019)

文章

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Protein modifications are crucial for disease study. Analysis methods are key.

蛋白質修飾對疾病研究至關重要。分析方法是關鍵。

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