推薦產品
product name
MDR1 Knockout Caco-2 Cells, one vial
生物源
human male colorectal tissue (Source disease: colon adenocarcinoma)
形狀
liquid
技術
drug transporter assay: suitable
permeability assay: suitable
OMIM登錄號
應用
ADME/TOX
儲存溫度
−196°C
基因資訊
human ... ABCB1(5243)
一般說明
The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 (MDR1) efflux transporter.
應用
The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
特點和優勢
The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:
- A functional knockout of the MDR1 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MDR1 substrate
- The 24 well Transwell format enables the MDR1 knockout cells to be included in standard drug transporter protocols
- Human assay with no interference from animal inhibitors
- Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality
免責聲明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Felix Huth et al.
Journal of pharmaceutical sciences, 110(6), 2562-2569 (2021-02-05)
The estimation of the extent of absorption of drug candidates intended for oral drug delivery is an important selection criteria in drug discovery. The use of cell-based transwell assays examining flux across cell-monolayers (e.g., Caco-2 or MDCK cells) usually provide
Angelo E Andres et al.
Methods in molecular biology (Clifton, N.J.), 2430, 449-466 (2022-04-28)
Taxoids such as paclitaxel (Taxol) are an important class of anticancer drugs that bind β-tubulin and stabilize cellular microtubules. To provide new chemical tools for studies of microtubules, we synthesized derivatives of paclitaxel modified at the 7-position with the small
P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship
文章
Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.
在 96 孔系統中使用 Millipore 的 Millicell-96 系統進行藥物轉運分析的應用說明。
Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.
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