MSANGERV

桑格全基因组 慢病毒 CRISPR 文库

Mouse, Virus Format

• 同義詞: Arrayed CRISPR library, Sanger CRISPR library
• 分類程式碼代碼: 41105904

屬性

• 品質等級: 200
• 包裝: pkg of 10 μL (384-well plate)
• 濃度: 1x10⁶ VP/ml (via p24 assay)
• 應用: CRISPR
• 運輸包裝: dry ice
• 儲存溫度: −70°C

描述

一般說明

两名基因组编辑领导者Sigma-Aldrich和Wellcome Trust Sanger Institute联手打造了第一个阵列化的慢病毒CRISPR基因敲除文库。基于文献中已验证的技术，Sanger CRISPR库将使您的实验室走在竞争的最前沿，以实现下一个重大发现。

應用

功能基因组学/筛选/靶标验证

特點和優勢

• Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
• Simplify the workflow with puromycin selection
• Illuminate CRISPR-expressing cells with BFP

Additional Features

• Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
• Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
• Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

包裝

384 well plates

成分

Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones


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外觀

10μl of lentivirus at ≥10⁶ particles/ml in ~120×384 well plates

其他說明

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

推薦產品

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Human, Virus Format

法律資訊

CRISPR Use License Agreement

Lentiviral License Agreement

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable