MAK504

Coenzyme A Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• 同義詞: Assay Kit for Coenzyme A Detection
• 分類程式碼代碼: 12352204
• NACRES: NA.84

屬性

• 品質等級: 100
• 檢測方法: colorimetric; fluorometric
• 相關疾病: endocrinological disorders, diabetes; cancer
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle, and many others. One of CoA′s most crucial roles is the carrying and transferring of acyl groups.

特點和優勢

• Linear detection range: 5 - 1000 µM for the colorimetric assay and 3 - 100 µM CoA for the fluorometric assay.
• Compatible with high-throughput handling systems.

適合性

The kit is suitable for Coenzyme A (CoA) determination in a variety of biological samples.

原則

The Coenzyme A Assay Kit provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the CoA concentration in the sample.

安全資訊

• 象形圖: GHS08,GHS05,GHS09
• 訊號詞: Danger
• 危險聲明: H318,H334,H410
• 防範說明: P261 - P273 - P280 - P284 - P305 + P351 + P338 - P391
• 危險分類: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3