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MAK461

pNPP Phosphatase Assay Kit

Name: <I>p</I>NPP Phosphatase Assay Kit
Brand: SIGMA

Sufficient for 500 colorimetric tests

同義詞:

Phosphatase Assay Kit, p-Nitrophenyl Phosphate Assay Kit

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NACRES:

NA.84

UNSPSC Code:

12161503

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屬性

Quality Segment

100

input

protein

detection method

colorimetric

relevant disease(s)

cancer; hematological disorder; gastrointestinal diseases; neurological disorders; cardiovascular diseases

storage temp.

−20°C

描述

General description

Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A₄₀₅ nm on a spectrophotometer.

Application

The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.

The pNPP Phosphatase Assay Kit may be used for:

Metabolism Research
Cardiovascular Research
Neurodegenerative Research

Biochem/physiol Actions

The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 °C.

Features and Benefits

Simplified Process: Enjoy a hassle-free procedure that can be completed within 30 minutes without wash or reagent transfer steps, saving you time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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本產品	CS0740	MAK447	MAK452
Sigma-Aldrich MAK461 pNPP Phosphatase Assay Kit	Sigma-Aldrich CS0740 酸性磷酸酶活性检测试剂盒	Sigma-Aldrich MAK447 碱性磷酸酶检测试剂盒	Sigma-Aldrich MAK452 Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit
description Sufficient for 500 colorimetric tests	description 1 kit sufficient for 1,000 assays (multiwell plates), 1 kit sufficient for 100 assays (tubes)	description Sufficient for 250 colorimetric tests	description Sufficient for 100 colorimetric tests
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 100
detection method colorimetric	detection method -	detection method colorimetric	detection method colorimetric
input protein	input -	input -	input -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
relevant disease(s) cancer; hematological disorder; gastrointestinal diseases; neurological disorders; cardiovascular diseases	relevant disease(s) -	relevant disease(s) osteoporosis; gastrointestinal diseases; orthopedic diseases; cancer	relevant disease(s) hematological disorder, anemia; cancer

圖示

GHS08,GHS05,GHS07,GHS09

警示詞

Danger

危險代碼

H302 + H332,H314,H341,H373,H411

預防性聲明

P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

靶向器官

Nervous system,Kidney,Liver,Skin

存儲類別/等級

8A - Combustible corrosive hazardous materials

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WGK 3

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Lot/Batch Number

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