MAK444

α-L-Fucosidase Assay Kit

Sufficient for 100 colorimetric tests

• 分類程式碼代碼: 12161503
• NACRES: NA.84

屬性

• 品質等級: 100
• 輸入: tissue; serum; plasma; cell lysate
• 檢測方法: colorimetric
• 相關疾病: cancer, diabetes; endocrinological disorders, hepatitis; gastrointestinal diseases
• 儲存溫度: 2-8°C

描述

一般說明

The enzyme α-L-Fucosidase (AFU), encoded by the FUCA1 gene in humans, is responsible for breaking down L-Fucose. Genetic deficiency in this enzyme leads to fucosidosis, a neurovisceral storage disease characterized by fucose accumulation. Reduced serum activity of fucosidase is associated with ovarian carcinoma, while elevated activity is linked to conditions such as diabetes, hyperthyroidism, cirrhosis, and hepatitis. Additionally, increased AFU activity has been observed in various types of carcinomas including lung, breast, stomach, ovary, uterus, and liver cancers.

應用

The α-L-Fucosidase Assay Kit may be used for:

• Fucosidosis Research
• Cancer Research
• Endocrinology Research

特點和優勢

• Efficient Process: Experience a simplified process with our kit, requiring only one working reagent and a 20-minute assay reaction, saving valuable time and effort in your workflow.
• Expanded Detection Capability: Detect α-ʟ-Fucosidase levels precisely across a wide range, from 1 to 100 U/L, using just 10 μL of sample in a 96-well plate, allowing for comprehensive analysis with minimal sample volume.
• Compatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring streamlined operations and precise results, enhancing efficiency in your laboratory workflow.

適合性

The kit is suitable for the quantitative determination of α-L-Fucosidase activity in plasma, serum, tissue, cell lysate, etc.

原則

The non-radioactive, colorimetric α-L-Fucosidase Assay Kit is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

其他說明

For additional information on our range of Biochemicals, please complete this form.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H319
• 防範說明: P264 - P280 - P305 + P351 + P338 - P337 + P313
• 危險分類: Eye Irrit. 2
• 儲存類別代碼: 10 - Combustible liquids