推薦產品
檢測方法
fluorometric
相關疾病
cancer; cardiovascular diseases
儲存溫度
−20°C
一般說明
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
特點和優勢
Cathepsin L Activity Assay Kit:
- Simple procedure with results in 1-2 hours.
適合性
The kit is suitable for the measurement of cathepsin L in cell lysates.
原則
The Cathepsin L Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin L will cleave the synthetic substrate FR-AFC to release free AFC. The released AFC can easily be quantified fluorometrically. The cathepsin L assay is simple and straightforward. Assay conditions have been optimized to obtain the maximal activity.
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
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