MAK387

Cathepsin B Activity Assay Kit

sufficient for 100 fluorometric tests

• 分類程式碼代碼: 12161503

屬性

• 檢測方法: fluorometric
• 相關疾病: cardiovascular diseases; cancer
• 儲存溫度: −20°C

描述

一般說明

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

特點和優勢

The Cathepsin-B Activity Assay kit:

• Is a simple one-step procedure with results in 1-2 hours.

適合性

The kit is suitable for the detection of Cathepsin-B activity in cell lysates.

原則

The Cathepsin-B Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate RR-AFC to release free AFC. The released AFC can easily be quantified using a fluorescence plate reader.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2