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M0535

Sigma-Aldrich

用于培养支原体的基础培养基

powder, suitable for microbiology

同義詞:

Mycoplasma media

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.74

形狀

powder

技術

microbiological culture: suitable

溶解度

H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)

應用

microbiology

一般說明

支原体肉汤是一种基础培养基,在用酵母提取物、马血清和抗生素富集后可支持支原体菌的生长。当用于培养支原体时,该基础培养基中所包含的成分不会表现出抑制或毒性作用。已添加了特殊的矿物质补充剂以改善生物体的生长和菌落特性。

應用

支原体肉汤可用于支原体的分离和培养。它在无氧条件可被用于培养 牙龈卟啉单胞菌

成分

粉末含有(g/L):10.0 细菌蛋白胨、10.0 LAB-LEMCO粉、5.0 氯化钠、0.5 矿物质补充剂。

準備報告

基础培养基:

1.将 25.5 g 培养基粉末溶于 1L 蒸馏水中。搅拌至粉末溶解

2.将溶液的 pH 值调至 7.8 ± 0.2.

3.在 121 °C 和 15 psi 下高温灭菌 15 分钟。冷却至50 °C并添加无菌补充剂。

儲存和穩定性

在室温下储存培养基粉末,在 2-8 °C 制备培养基。

其他說明

此产品仅供实验室使用。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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文章

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

透過 PCR、DNA 染色或培養測試,檢測細胞培養中的支原體污染。探索支原體預防、消除和檢測試劑盒。

Overview of good cell banking practices for cell line cryopreservation purposes.

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

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