M0195

MAPKAPK2

recombinant, expressed in HEK 293 cells, ≥60 units/mg protein, buffered aqueous glycerol solution

• 同義詞: MAP kinase activated protein kinase 2, MAPKAP Kinase 2 human
• MDL號碼: MFCD04974881
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 重組細胞: expressed in HEK 293 cells
• 形狀: buffered aqueous glycerol solution
• 比活性: ≥60 units/mg protein
• 分子量: 45-60 kDa
• UniProt登錄號: P49137
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... MAPKAPK2(9261)

描述

生化／生理作用

MAPKAPK2 is a serine/threonine protein kinase activated by the p38 MAP kinase in response to various signals including mitogenic and stress stimuli. MAPKAPK2 is distinguished from the RSK family of ribosomal protein S6 kinases (also termed MAPKAPK1), which are activated by the MAP kinases, ERK1, and ERK2 based on its substrate specificity and amino acid sequence. Upon activation, p38 MAP kinase activates the downstream MAPKAPK2 by phosphorylation of the threonine residue in the Pro-Thr motif in response to heat shock or mitogenic stimuli. Activated MAPKAPK2 phosphorylates the small heat shock protein HSP25/HSP27, both in vitro and in vivo, at serine residues. Thus, HSP25/HSP27 seems to be the physiological substrate of MAPKAPK2.

單位定義

One unit will incorporate 1.0 nmole of phosphate into HSP 25 per minute at 30 °C at pH 7.4.

外觀

Solution in 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 0.05 mM EDTA, 2 mM DTT, and 10% glycerol.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable