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Merck
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H0537

Millipore

HIS-Select® HF 镍亲和凝胶

同義詞:

Ni-NTA HF resin

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

形狀

suspension

品質等級

200

技術

affinity chromatography: suitable

基質

Highly cross-linked 6% Beaded Agarose

容量

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

儲存溫度

2-8°C

相關類別

一般說明

HIS-Select 高流动性镍亲和凝胶是一种固定化金属离子亲和色谱（IMAC）凝胶，设计用于特异性结合色谱系统中的His-tag蛋白（含组氨酸蛋白质），压力高达 200 psi，最大线性流速为 3,000 cm/hr。

應用

亲和凝胶适用于在天然和变性条件下纯化组氨酸标记的蛋白质（His-tag蛋白）。
镍亲和凝胶用于纯化His-tag® 蛋白（组氨酸标记蛋白） 。 HIS-Select® 高流动性 (HF) 产品将HIS-Select 技术优良的选择性和高度交联的琼脂糖相结合，可在压力下实现更高的流速和机械稳定性。 HIS-Select HF产品设计用于生产规模的纯化和FPLC™ 应用。 和其他HIS-Select产品一样，次氮基三乙酸镍（Ni-NTA)）的螯合基团与琼脂糖的不带电亲水键可确保实现真正的一步纯化。

特點和優勢

  • 高选择性确保了高纯度
  • 独特的不带电亲水键减少了非特异性结合
  • 组氨酸标记蛋白（His-tag蛋白）的结合能力大于 15 mg/mL
  • 高度交联的琼脂糖基质允许在更高压力下纯化
  • 在变性或非变性条件下结合
  • 一步纯化

外觀

30% 乙醇溶液的混悬液 (1:1)

準備報告

使用前必须除去乙醇。用去离子水洗涤亲和凝胶以除去乙醇，然后用提供的平衡缓冲液进行平衡。

法律資訊

FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2

標靶器官

Eyes,Central nervous system, Respiratory Tract

水污染物質分類（WGK）

WGK 3

閃點（°F）

88.0 °F

閃點（°C）

31.1 °C

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

分析證明 (COA)

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