GE17-5286-01

HisTrap^™ Fast Flow Crude

Cytiva 17-5286-01, pack of 5 × 5 mL

• 同義詞: Histidine-tagged liquid chromatography column, FF crude columns, IMAC columns, protein purification columns
• 分類程式碼代碼: 23151816
• NACRES: NA.56

屬性

• 包裝: pack of 5 × 5 mL
• 製造商／商標名: Cytiva 17-5286-01
• 參數: <20 mL/min flow rate; 42 psi (H₂O at room temperature.)
• 床尺寸: 16 mm × 25 mm
• 床體積: 5 mL
• 柱內徑: 16 mm
• 基質: 6% cross-linked agarose
• 粒徑: 45-165 μm
• 平均直徑: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• 工作範圍: 3-12(Ni²⁺-stripped medium.)
• 容量: ~40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.); ~40 mg binding capacity(histidine-tagged protein)
• 適合性: suitable for bioprocess medium

描述

一般說明

HisTrap^™ FF crude columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysate by immobilized metal ion affinity chromatography (IMAC).

HisTrap^™ FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow. This prepacked column is intended for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). After thorough cell disruption, it is possible to load the unclarified lysate on the column without precentrifugation and filtration of the sample. Extending the duration of the mechanical treatment of the sample to ensure a more complete lysis is recommended. For optimal results, we recommend first addition of lysozyme and DNase I followed by a mechanical lysis, for example by sonication. The homogenized sample can then be loaded directly on the column without a clarification step which may prevent degradation of the target protein and increase the activity.

Ni Sepharose^™ 6 Fast Flow has low nickel ion (Ni²⁺) leakage and is compatible with a wide range of additives used in protein purification. The special design of the column in combination with the medium, provide fast, simple, and convenient purifications. Short purification time generally minimizes deleterious effects, such as degradation and oxidation of sensitive target proteins, and is therefore of great importance. HisTrap^™ FF crude columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design chromatography systems.

特點和優勢

• Optimized for purification of histidine-tagged proteins directly from homogenized, unclarified cell lysates.
• High binding capacity, approx. 40 mg/mL medium and negligible Ni²⁺ leakage
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Reduced sample preparation time, which minimizes risk of protein degradation by proteases.
• No need for centrifugation or filtration of the samples. Direct sample application.
• Reliable purification of histidine-tagged proteins directly from unclarified lysates–simply sonicate and run.
• Reduced sample preparation time, which minimizes risk of protein degradation by proteases
• Simple operation with a syringe, pump, or chromatography system such as AKTAdesign or FPLC^™ System
• Permit rapid yet reliable separations with a minimum of sample preparation and equipment

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

FPLC is a trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS09
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids