推薦產品
ligand
glutathione and 10-carbon linker arm
包裝
pack of 500 mL
製造商/商標名
Cytiva 17-5132-03
基質
highly cross-linked 4% agarose
平均直徑
90 μm
cleaning
3-12
工作範圍
3-12
容量
>10 mg/mL binding capacity
一般說明
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
特點和優勢
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
文章
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
條款
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
本頁說明如何使用 Cytiva 的 Glutathione Sepharose 純化 GST 標記蛋白。
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
GSTrap™ 親和柱是特別設計的 1 ml 和 5 ml HiTrap® 柱,使用 Glutathione Sepharose High Performance、Glutathione Sepharose 4 Fast Flow 或 Glutathione Sepharose 4B 媒介包裝。
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