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GE17-5131-01

GSTrap™ Fast Flow

Cytiva 17-5131-01, pack of 1 × 5 mL

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About This Item

分類程式碼代碼:
23151817
NACRES:
NA.25

ligand

glutathione and 10-carbon linker arm

包裝

pack of 1 × 5 mL

製造商／商標名

Cytiva 17-5131-01

儲存條件

(20% Ethanol)

參數

<20 mL/min flow rate
42 psi

床尺寸

16 mm × 25 mm

床體積

5 mL

柱內徑

16 mm

基質

highly cross-linked 4% agarose

平均直徑

90 μm

cleaning

3-12

工作範圍

3-12

容量

>10 mg/mL binding capacity

儲存溫度

2-8°C

一般說明

GSTrap™ FF columns are prepacked Glutathione Sepharose™ Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

應用

GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System.

特點和優勢

  • Fast and simple one-step purification of GST-tagged Proteins.
  • Mild elution conditions preserving Protein antigenicity and function.
  • Easy scale-up by connecting the columns in series.
  • Sepharose™ 4 Fast FLow provides good fLow properties.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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文章

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

條款

HiTrap® Benzamidine FF for Thrombin & Factor Xa Removal

Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

使用 GST HiTrap® 1 mL 和 5 mL 色譜柱進行純化：GSTrap™ 4B、GSTrap™ FF 和 GSTrap™ HP

GSTrap™ 親和柱是特別設計的 1 ml 和 5 ml HiTrap® 柱，使用 Glutathione Sepharose High Performance、Glutathione Sepharose 4 Fast Flow 或 Glutathione Sepharose 4B 媒介包裝。

以酶切方式移除 GST Tag

本頁說明如何使用 Cytiva 產品以酵素裂解方式移除 GST 標籤。

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相關內容

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.

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