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G8420

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

同義詞:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in E. coli overproducing strain

品質等級

形狀

lyophilized powder

比活性

≥20,000 units/mg protein

分子量

290 kDa

成份

, ~25%

儲存溫度

−20°C

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相關類別

應用

比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据,关于使用不同来源的酶优化不同药物类别的水解,并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。

品質

含有缓冲盐和稳定剂。

單位定義

一个Sigma或改良的Fishman单位将在37℃,pH6.8(30分钟测定)下每小时从酚酞葡糖苷酸中释放1.0μg酚酞。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Enzymes
Hans Ulrich Bergmeyer
Methods of Analysis, 2, 460-461 (1974)
V Graef et al.
Clinical chemistry, 23(3), 532-535 (1977-03-01)
We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the
Amichay Meirovitz et al.
The FEBS journal, 280(10), 2307-2319 (2013-02-13)
Recent years have seen a growing body of evidence that enzymatic remodeling of heparan sulfate proteoglycans profoundly affects a variety of physiological and pathological processes, including inflammation, neovascularization, and tumor development. Heparanase is the sole mammalian endoglycosidase that cleaves heparan
Shu Feng et al.
Cancer research, 73(8), 2551-2562 (2013-02-27)
Prostate cancer is the most common visceral malignancy and the second leading cause of cancer deaths in US men. There is broad evidence that fibroblast growth factor (FGF) receptors are important in prostate cancer initiation and progression, but the contribution
Xiaoyan Liu et al.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 42(1), 67-74 (2013-03-19)
To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP

文章

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

條款

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

相關內容

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

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