G115050

DMT-dG(dmf) Phosphoramidite

configured for ABI, configured for (ÄKTA^® and OligoPilot^®)

• 同義詞: DMT-dG(dmf) Amidite, N-[（二甲基氨基）亚甲基]-5′-O- [双（4-甲氧基苯基）苯基甲基]-2′-脱氧鸟苷,3′-[2-氰基乙基N,N-双（1-甲基乙基）亚磷酰胺], N2-二甲基甲脒基-5′-O-（4,4′-二甲氧基三苯甲基）-2′-脱氧鸟苷-3′-O-[O-（2-氰基乙基）-N,N′-二异丙基亚]
• 經驗公式（希爾表示法）: C₄₃H₅₃N₈O₇P
• CAS號碼: 330628-04-1
• 分子量：: 824.90
• MDL號碼: MFCD00797420
• 分類程式碼代碼: 12352200
• PubChem物質ID: 329799825
• NACRES: NA.51

屬性

• 種類: for DNA synthesis
• 產品線: Proligo Reagents
• 化驗: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• 形狀: powder
• 技術: oligo synthesis: suitable
• 顏色: white to off-white
• &lambda ；: conforms (UV/VIS Identity)
• 相容性: configured for (ÄKTA^® and OligoPilot^®); configured for ABI
• 核苷譜: base: deoxyguanosine; base protecting group: DMF; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast/standard
• 儲存溫度: -10 to -25°C
• SMILES 字串: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(\N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1
• InChI 密鑰: YRQAXTCBMPFGAN-UJASEYITSA-N

描述

一般說明

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Changing the dG protecting group to the dimethylformamide (dmf) base protecting group enables the rapid synthesis of high-purity, high-yieldoligonucleotide, thus increasing the efficiency of high-throughputproduction.

Key Features of dG(dmf):

• dG(dmf) is deprotected faster than the conventional dG(ib): thedeprotection time in concentrated ammonia is reduced to 2 hours at55 °C or 1 hour at 65 °C
• The dG(dmf)-monomer is especially suitable for G-rich sequences:incomplete deprotection is greatly reduced in comparison with theconventional dG(ib)-monomer
• dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-,dC(bz)- and dT-phosphoramidites
• dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
• No change is required in the reagents commonly used for DNA synthesis(except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should beemployed)DMT-dG(dmf) Phosphoramidite is configured for ABI^™ Synthesizers.

法律資訊

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable