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CS0010

β-分泌酶(BACE1) 活性检测试剂盒（荧光法）

Name: β-分泌酶(BACE1) 活性检测试剂盒（荧光法）
Brand: SIGMA

1 kit sufficient for 250 reactions

同義詞:

BACE1 活性检测试剂盒（荧光法）

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關於此項目

NACRES:

NA.84

UNSPSC Code:

12161503

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屬性

Quality Segment

300

usage

kit sufficient for 250 reactions

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... BACE1(23621)

描述

Application

该试剂盒包含有效检测BACE1活性所需的全部试剂。其中包含筛选潜在BACE1抑制剂的酶。该检测基于荧光共振能量转移 (FRET) 技术，即在BACE1切割底物后观测增强的荧光信号。

Biochem/physiol Actions

作为一种跨膜蛋白酶，BACE1负责切割淀粉样前体蛋白 (APP) 的β位点来产生β淀粉样肽 (Aβ)。大脑中Aβ积累是阿尔茨海默氏症的主要病因。BACE1是抑制剂药物研发的靶点。

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本產品	S4195	RAB0623	MAB5308
Sigma-Aldrich CS0010 β-分泌酶(BACE1) 活性检测试剂盒（荧光法）	Sigma-Aldrich S4195 β-分泌酶人	Sigma-Aldrich RAB0623 人BACE1 /β-分泌酶1 ELISA试剂盒	Sigma-Aldrich MAB5308 抗-BACE抗体，CT，克隆61-3E7
description 1 kit sufficient for 250 reactions	description recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), ≥10,000 units/mg protein	description -	description clone 61-3E7, Chemicon^®, from mouse
usage kit sufficient for 250 reactions	usage -	usage -	usage -
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. -
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
Gene Information human ... BACE1(23621)	Gene Information human ... BACE1(23621)	Gene Information human ... BACE1(23621)	Gene Information human ... BACE1(23621)
Quality Level 300	Quality Level 200	Quality Level -	Quality Level 100

僅套裝組件

產品號碼

描述

Fluorescent Assay Buffer 50 mL

Stop Solution 15 mL

Substrate (MOCA-SEV-NL-DAEFR-DNP-RR) 500 μL

Assay Standard 140 μL

BACE1 (β−Secretase) 300 units 100 μL

存儲類別/等級

10 - Combustible liquids

wgk

WGK 3

從最近期的版本中選擇一個：

分析證明 (COA)

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Questions

1–2 of 2 Questions

3 months ago

What is the PDB ID of enzyme used?

1 answer
1. Technical Support
  
  a month ago
  
  This kit includes human BACE1/?-secretase enzyme for activity detection and inhibitor screening. The exact PDB ID for the enzyme is not specified, however the protein is identical to product S4195, UniProt P56817. Please see the links below to review information that may be helpful.
  UniProt:
  https://www.uniprot.org/uniprotkb/P56817/entry
  RCSB PDB page for UniProt P56817
  https://www.rcsb.org/groups/sequence/polymer_entity/P56817
  Also note that commonly cited human BACE1 structures include PDB IDs 2ZHT and 1W50, References:
  PDB ID 2ZHT: Human BACE1 crystal structure — https://www.rcsb.org/structure/2ZHT
  PDB ID 1W50: Human BACE apo structure — https://www.rcsb.org/structure/1W50
  
  Helpful?
2 years ago

what is the concentration of BACE1 enzyme in micromolar?

1 answer
1. Technical Support
  
  2 years ago
  
  The B9059 BACE-1 product included in the kit, serves as a positive control. The Technical Bulletin notes that the concentration of this item is approximate 3 units/microliter. This material has not been tested for purity. Therefore, a molar concentration cannot be provided.
  
  Helpful?

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β-分泌酶(BACE1) 活性检测试剂盒（荧光法）

1 kit sufficient for 250 reactions