CRISPR16H

Lenti CRISPR HPRT1 gRNA Control Transduction Particles, High Titer (LV02 vector)

• 分類程式碼代碼: 41106609
• NACRES: NA.51

屬性

• 形狀: liquid
• 品質等級: 200
• 包裝: vial of 8x25 μL (aliquots)
• 濃度: (5x10⁸ TU/ml (via p24 titering assay))
• 應用: CRISPR
• 運輸包裝: dry ice
• 儲存溫度: −70°C

描述

一般說明

The Lenti CRISPR HPRT1 gRNA Control Transduction Particles (LV02 vector) product is a critical positive control used to monitor transduction efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control uses a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and Ef1a-driven Puromycin resistance casette. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

應用

功能性基因组学研究/筛选/靶标验证

其他說明

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

法律資訊

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable