CLL1033

U2OS GFP-LMNB1

human female bone (Disease source: Osteosarcoma)

• 分類程式碼代碼: 41106514
• NACRES: NA.81

屬性

• product name: U2OS GFP-LMNB1,
• 生物源: human female bone (Source Disease: Osteosarcoma)
• OMIM登錄號: 169500
• 儲存溫度: −196°C
• 基因資訊: human ... lamin B1(4001)

描述

一般說明

LMNB1 (lamin B1) is a B type lamin protein. LMNB1 is abundantly expressed in various cell types. This gene is located on human chromosome 5q23. The nuclear lamina, an intermediate filament meshwork, is made of LmnB1, LmnB2, A type (LmnA/C) and associated inner nuclear membrane proteins.

This product was derived from ATCC Cat. No. HTB-96.

U2OS GFP-LMNB1 are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification.

應用

This product is a human U2OS cell line in which the genomic LaminB1 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr^® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of LaminB1. Fluorescence imaging shows the characteristic LaminB1 nuclear expression. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.

To learn more, please visit the Cellular Reporter Cell Line webpage

生化／生理作用

Lamin B1 (LMNB1) is essential for proper chromosome condensation in interphase nuclei. It also participates in the distribution of heterochromatin and modulation of gene expression and splicing. Mutations in LMNB1 (lamin B1) gene results in the adult onset demyelinating disease autosomal dominant leukodystrophy (ADLD).

Nuclear lamin B1 encoded by LMNB1 gene is an intermediate filament protein of the nuclear envelope. The protein has transcriptional co-regulatory activity. It plays a key role in DNA replication, cellular aging and stress responses. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC) patients. Thus, LMNB1 can be used as a potential biomarker for HCC.

特點和優勢

GFP-tagged on chromosome 5q23.2

The U2OS cells are adherent, with a doubling time of approx. 29 hours.

成分

U2OS GFP-LMNB1 cells

品質

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

準備報告

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:6

The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164).

法律資訊

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable