SHP-77

98110201, human lung, Not specified

• 同義詞: Human SHP-77, SHP-77 Cell Line
• 分類程式碼代碼: 41106514

屬性

• product name: SHP-77, 98110201
• 生物源: human lung
• 增長模式: Adherent/aggregates in suspension
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian lung small cell carcinoma, large cell variant

細胞系描述

SHP-77 was established in 1977 being derived from a non-encapsulated primary lung tumor from the apical portion of the upper lobe of the left lung. The patient was a 54 year old male with blood type O, Rh+. The cell line has a variety of unusual characteristics including the morphology of an undifferentiated large cell variant of small cell lung carcinoma (SCLC) but biochemical properties of a classical SCLC. SHP-77 express neural cell adhesion molecule (NCAM), NHK-1, CD56 and the cell surface leucocyte antigen Leu-7(HNK-1;CD57). Other neuroendocrine markers are L-dopa decarboxylase and dense core secretory granules. Gland formation and intracytoplasmic lamellar bodies have been shown by electron microscopy. This cell line is useful as in vitro target in 51Cr and III In release cytotoxicity assays. They are suitable for in vitro nude mice assays for evaluation of immune reactivity of cells and serum from lung cancer patients. In addition, they serve to evaluate the immune status of patients with SCLC who received radiation or chemotherapy treatment. SHP-77 cells are tumorigenic in nude mice and grow as circumscribed nodules without metastases formation. They have a reported doubling time of 96 hours.

應用

SCLC studies, cytotoxicity assays, immunological studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8
D16S539: 11
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 9,11
vWA: 16

培養基

RPMI 1640 + 2mM Glutamine + 10% Heat Inactivated Foetal Bovine Serum (HI-FBS).

例行更新培養

Maintain cultures between 4-9 x 100,000 cells / ml; 5% CO₂; 37°C. Cells grow as mixture of loosely adherent and floating clusters.

其他說明

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