293-PERV-PK-CIRCE

97051411, human kidney, Not specified

• 分類程式碼代碼: 41106514

屬性

• product name: 293-PERV-PK-CIRCE, 97051411
• 生物源: human kidney
• 增長模式: Adherent aggregates
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human embryo kidney, infected with endogenous retrovirus of pigs

細胞系描述

293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.

應用

Pig retrovirus studies, infectivity assays

培養基

DMEM + 2mM Glutamine + 10% HI-FBS

例行更新培養

Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO₂ 3

其他說明

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