EMT6/VRP/R

96042319, mouse unknown/unspecified, Epithelial-like

• 分類程式碼代碼: 41106514

屬性

• product name: EMT6/VRP/R, 96042319
• 生物源: mouse unknown/unspecified
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse mammary, drug-resistant

細胞系描述

The drug-resistant line EMT6/VRP/R was established from the parent cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in verapamil (see reference).

應用

Drug-resistance studies

培養基

MEM (EBSS) +2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 75 μg/ml verpamil + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37ºC. It is recommended to resuscitate cells in drug-free medium. Add drug after growth is established.

其他說明

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