MES-SA/Dx-5

95051031, human uterus, Epithelial

• 分類程式碼代碼: 41106514
• eCl@ss: 32190102

屬性

• product name: MES-SA/Dx-5, 95051031
• 生物源: human uterus
• 增長模式: Adherent
• 染色體組型: Modal no. 47 (parent line MES-SA-45). Reported karyotype 47,XX,12qt,22q-t(5q,6p),-3,-4,-7,+t(3q?,7q),Mar1,+mar2,+Mar3
• 形態學: Epithelial
• 產品: High levels of Mdr1 mRNA and P-glycoprotein
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human uterus sarcoma

細胞系描述

The multi drug-resistant cell line MES-SA/Dx5 was derived from the human uterine sarcoma cell line MES-SA (Sigma Catalogue number. 95051030) which was originally obtained from a tumour from a 56-year-old Caucasian female at the time of hysterectomy. The Dx5 variant exhibits a 100-fold resistance to doxorubicin and has a reported doubling time of 30 hours. The two additional marker chromosomes indicate clonal selection during drug selection. MES-SA/Dx-5 cells exhibit marked cross-resistance to a number of chemotherapeutic agents (including daunorubicin, dactinomycin, vincristine, taxol, colchicine) and moderate cross-resistance to mitomycin C and melphalan. Cross resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.

應用

Tumour toxicity assays and multi-drug-resistant studies.

培養基

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.03% EDTA; 5% CO₂; 37°C. Tap side of flask to dislodge cells.

其他說明

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