46BR.1N

92100623, human skin, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: 46BR.1N, 92100623
• 生物源: human skin
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human skin fibroblast

細胞系描述

Derived from 46BR, a fibroblast from an individual with hypogammaglobulinaemia. The parent cell line was transformed with the plasmid pSV3neo expressing SV40 T-antigen and is now immortal.

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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