CFPAC-1

91112501, human pancreas (liver metastasis), Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: CFPAC-1, 91112501
• 生物源: human pancreas (liver metastasis)
• 增長模式: Adherent
• 染色體組型: Hyperdiploid, (65-75 chromosomes)
• 形態學: Epithelial
• 產品: Not specified
• 受體: Cystic fibrosis trans-membrane regulator (CFTR)
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian pancreatic adenocarcinoma

細胞系描述

CFPAC-1 is a ductal pancreatic adenocarcinoma derived by differential trypsinisation of explant cultures from a metastatic lesion in the liver of a 26 year old caucasian male with cystic fibrosis (CF). The cell line expresses cystic fibrosis trans-membrane regulator (CFTR) and ion transport properties consistent with the CF defect. CFPAC-1 cells show an epithelial morphology and polarisation with apical microvilli. Expression of cytokeratin and oncofoetal antigens characteristic of pancreatic duct cells have been described. The cells express the CF gene and show the most common CF mutation, deletion of three nucleotides result in a phenylalanine-508 deletion. CFPAC-1 cells are also tumorigenic in nude mice.

應用

Cystic fibrosis studies, genetic analysis

培養基

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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