Detroit 573

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 分類程式碼代碼: 41106514

屬性

• 生物源: human skin
• 包裝: tube of 5 μg 90112601-DNA-5UG; pkg of vial of cells 90112601-1VL
• 增長模式: Adherent
• 染色體組型: Aneuploid
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian skin, B/D translocation

細胞系描述

Detroit 573 was initiated in 1967 from a skin biopsy of a deceased new-born female with multiple congenital anomalies. Approximately 94% of the cell population is 45,XX,t(Bp-;Dq+). The translocated B/D chromosome morphologically resembles a group A-2 chromosome. The cell line is susceptible to polyvirus type 1 and vesicular stomatitis (Indiana Strain) virus.

應用

Karyology studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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