NCTC 2071

90050803, mouse connective tissue, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: NCTC 2071, 90050803
• 生物源: mouse connective tissue
• 增長模式: Adherent
• 染色體組型: Modal no. 63
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse C3H/An connective tissue

細胞系描述

Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.

應用

Nutritional, immunological and toxicological studies

培養基

NCTC 135 100% + 2mM Glutamine.

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm² by scraping off the cells and resuspending into new flasks; 5% CO₂; 37°C. Freeze in 9% glycerol/91% FBS.

其他說明

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