NIH-3T3D4

85111801, mouse embryo, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: NIH-3T3D4, 85111801, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• 生物源: mouse embryo
• 包裝: tube of 5 μg 85111801-DNA-5UG; pkg of vial of cells 85111801-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

一般說明

Product has lost contact inhibition

細胞系來源

Mouse Swiss NIH embryo

細胞系描述

Derived from the NIH-3T3 cell line. No longer exhibit contact inhibition.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2x10,000 to 2x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.