CHO Cell Line from Chinese hamster ovary

85050302, hamster ovary, Epithelial

• 同義詞: CHO Cells, CHO-ori Cells, Chinese Hamster Ovary Cells, Chinese hamster ovary cell line
• 分類程式碼代碼: 41106514

屬性

• 產品名稱: CHO Cell Line from Chinese hamster ovary, 85050302, from hamster ovary
• 生物源: hamster ovary
• 存貨情形: not available in Japan
• 增長模式: Adherent
• 染色體組型: Hypodiploid, modal no. 20
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Hamster Chinese ovary

細胞系描述

A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

應用

CHO Cell Line from Chinese hamster ovary has been used:

• to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
• to carry out the vaginolysin (VLY) cytolytic activity assay
• as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

Nutritional and gene expression studies

培養基

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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