NS20Y-BU-4

8062503, mouse nerve, Neuronal

• 分類程式碼代碼: 41106514

屬性

• product name: NS20Y-BU-4, 08062503
• 生物源: mouse nerve
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Neuronal
• 產品: Choline acetyltransferase
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse neuroblastoma

細胞系描述

A bromodeoxyuridine-resistant mutant of NS20Y (Sigma Catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 Mouse strain A/Jax.

應用

Used in the generation of cell hybrids.

培養基

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridine

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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