PNV35B

01080924, Reactivity: Anti-Osteoclasts (human foetal), ECV304 and U937 cells

• 分類程式碼代碼: 41106514

屬性

• ImmunoDon: BALB/c mice
• 骨髓瘤: Ag876
• 反應: Anti-Osteoclasts (human foetal), ECV304 and U937 cells
• 技術: cell culture | mammalian: suitable
• 同型: IgM plus IgG
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Anti-Osteoclastoma Hybridoma

細胞系描述

135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.

免疫原

Osteoclastoma tumour

應用

Immunocytochemistry

培養基

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) + HT Supplement (H0137)

例行更新培養

Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO₂, 37°C,

其他說明

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