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Merck
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重要文件

B8781

Sigma-Aldrich

Restriction Enzyme Buffer SB

10 ×

同義詞:

Restriction Endonuclease Buffer

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.53

等級

for molecular biology

品質等級

描述

10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl2, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.

形狀

buffered aqueous solution

濃度

10 ×

儲存溫度

−20°C

SMILES 字串

CC(C)CC(CNC(Cc1ccccc1)C(N)=O)NC(=O)C(Cc2cnc[nH]2)NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)C(Cc3c[nH]c4ccccc34)NC(=O)C(CCC(N)=O)NC(=O)C(N)Cc5c[nH]c6ccccc56)C(C)C

InChI

1S/C58H77N15O9/c1-32(2)21-39(29-65-46(52(61)76)22-35-13-7-6-8-14-35)69-57(81)48(25-38-28-62-31-67-38)70-50(75)30-66-58(82)51(33(3)4)73-53(77)34(5)68-56(80)47(24-37-27-64-44-18-12-10-16-41(37)44)72-55(79)45(19-20-49(60)74)71-54(78)42(59)23-36-26-63-43-17-11-9-15-40(36)43/h6-18,26-28,31-34,39,42,45-48,51,63-65H,19-25,29-30,59H2,1-5H3,(H2,60,74)(H2,61,76)(H,62,67)(H,66,82)(H,68,80)(H,69,81)(H,70,75)(H,71,78)(H,72,79)(H,73,77)

InChI 密鑰

YNZJHLFHFNCZIE-UHFFFAOYSA-N

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一般說明

This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.

應用

Restriction Enzyme Buffer SB has been used in restriction digestion of PCR amplified product.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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存取文件庫

Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+ C nucleotide content and secondary structure melting point
Bukovska P, et al.
Journal of Microbiological Methods, 82(3), 223-228 (2010)

文章

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

限制酵素」一詞起源於 Werner Arber 和 Matthew Meselson 實驗室對腸細菌噬菌體 λ (lambda phage) 的研究。

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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