推薦產品
生物源
mouse
品質等級
共軛
peroxidase conjugate
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
PT-66, monoclonal
形狀
lyophilized powder
包裝
vial of 0.2 mL conjugate
技術
direct ELISA: 1:60,000 using Phosphotyrosine-BSA
dot blot: 1:40,000-1:200,000 using phosphotyrosine-BSA using chromogenic and chemiluminescent substrates, respectively
同型
IgG1
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
经 ELISA 和竞争性 ELISA 确定，该抗体与磷酸化的酪氨酸特异性反应，该磷酸化的酪氨酸既可以是游离氨基酸形式，也可与 BSA 或 KLH 等载体结合的形式。与非磷酸化的酪氨酸、磷酸苏氨酸、磷酸丝氨酸、AMP 或 ATP 没有观察到交叉反应。
Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the PT-66 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a phosphotyrosine-BSA conjugate.
特異性
Mouse anti-phosphotyrosine-peroxidase antibody reacts specifically with phosphorylated tyrosine coupled to BSA. The product has shown no reactivity for other phosphorylated amino acids like phosphothreonine and phosphoserine.
免疫原
与 BSA 结合的磷酸酪氨酸
應用
Monoclonal Anti-Phosphotyrosine-Peroxidase antibody has been used in
- enzyme linked immunosorbent assay (ELISA)
- dot blot
- Chemiluminescence dot blot
- kinase assay
生化／生理作用
Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine–peroxidase antibody can be used in kinase assay to visualize the fraction of phosphorylated substrate.
外觀
Lyophilized from a solution containing 1% bovine serum albumin and 0.05% MIT in 0.01 M sodium phosphate buffered saline.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Identification and biophysical assessment of the molecular recognition mechanisms between the human haemopoietic cell kinase Src homology domain 3 and ALG-2-interacting protein X
Shi X, et al.
The Biochemical Journal, 431(1), 93-102 (2010)
Bobbi Xayarath et al.
Journal of bacteriology, 189(9), 3369-3381 (2007-02-27)
Extracellular polysaccharides of many bacteria are synthesized by the Wzy polymerase-dependent mechanism, where long-chain polymers are assembled from undecaprenyl-phosphate-linked repeat units on the outer face of the cytoplasmic membrane. In gram-positive bacteria, Wzy-dependent capsules remain largely cell associated via membrane
Yuko Naito et al.
Molecular and cellular biology, 27(8), 3008-3022 (2007-02-14)
Sialic acid (Sia) is a family of acidic nine-carbon sugars that occupies the nonreducing terminus of glycan chains. Diversity of Sia is achieved by variation in the linkage to the underlying sugar and modification of the Sia molecule. Here we
Xiaoli Shi et al.
The Biochemical journal, 431(1), 93-102 (2010-07-31)
SFKs (Src family kinases) are central regulators of many signalling pathways. Their functions are tightly regulated through SH (Src homology) domain-mediated protein-protein interactions. A yeast two-hybrid screen using SH3 domains as bait identified Alix [ALG-2 (apoptosis-linked gene 2)-interacting protein X]
Ana Izabel Silva Balbin Villaverde et al.
Molecular & cellular proteomics : MCP, 19(11), 1860-1875 (2020-08-26)
After ejaculation, mammalian spermatozoa must undergo a process known as capacitation in order to successfully fertilize the oocyte. Several post-translational modifications occur during capacitation, including sialylation, which despite being limited to a few proteins, seems to be essential for proper
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