推薦產品
生物源
goat
共軛
peroxidase conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
物種活性
human
技術
direct ELISA: 1:40,000
dot blot: 1:100,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:100,000
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
一般說明
人IgG是糖蛋白抗体,含有两条相等的轻链和一对相同的重链。 IgG具有四种不同亚型,范围从IgG1到IgG4。这些抗体可调节机体对过敏和病原性感染的免疫反应。IgG还与补体结合和自身免疫疾病有关。
通过免疫特异性纯化从抗人IgG抗血清中分离抗体,该过程可基本上去除所有山羊血清蛋白,包括免疫球蛋白(其不可与人IgG的Fab片段特异性结合)。将抗人IgG与过氧化物酶缀合,然后进一步纯化以除去未缀合的物质。
通过免疫特异性纯化从抗人IgG抗血清中分离抗体,该过程可基本上去除所有山羊血清蛋白,包括免疫球蛋白(其不可与人IgG的Fab片段特异性结合)。将抗人IgG与过氧化物酶缀合,然后进一步纯化以除去未缀合的物质。
免疫原
人IgG的Fab片段
應用
山羊抗人IgG(Fab特异性)- 过氧化物酶抗体可用于直接ELISA(1:40,000)、斑点印迹和免疫组织化学(福尔马林固定,石蜡包埋切片;1:200)等应用。
山羊源抗人IgG(Fab特异性)-过氧化物酶抗体已用于以下应用:
在PBS中以1:10,000稀释抗人IgG抗体,并在室温下温育1小时。使用O-亚苯基-二胺底物溶液(Sigma)检测ELISA反应体系中的结合情况。
- 蛋白质印迹
- 直接酶联免疫吸附测定(ELISA)(1:40,000稀释),以分析产生重组Fab片段的转基因酵母培养物。
- 免疫组化
- 斑点杂交
- 免疫组化(福尔马林固定石蜡包埋切片,1:200)
在PBS中以1:10,000稀释抗人IgG抗体,并在室温下温育1小时。使用O-亚苯基-二胺底物溶液(Sigma)检测ELISA反应体系中的结合情况。
外觀
含0.05%MIT的0.01 M磷酸盐缓冲盐溶液(pH 7.4)
準備報告
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand.J. Immunol., 8, Suppl. 7, 7 (1978).
免責聲明
除非我们的产品目录或产品随附的其他公司文档中另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。
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訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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檢視決定選擇第二抗體的關鍵因素。瞭解種類、亞類、同種型、標籤等資訊。
Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.
我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.
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