WGA polymerase is compatible with TA cloning with the following alteration to the PCR step: Be sure to include a 7 to 30 minute extension at 72°C after the last cycle to ensure that all PCR products are full length and 3´ adenylated.
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一般說明
特點和優勢
- 以最少的模板获得更高的产量: 在不到 3 小时内将纳克量的基因组 DNA 扩增为微克产量(约 10μ g)
- 纳克样品可在 –20 °C 下保存以备将来使用
- 可选择各种DNA来源:全血、口腔拭子、血卡、植物、土壤、&福尔马林固定石蜡包埋的组织(FFPE)
- 全基因组扩增 (WGA) DNA 聚合酶提高扩增准确度
- 全基因组无可检出的等位基因偏倚
- 兼容许多下游应用,例如 TaqMan ®检测、单核苷酸多态性 (SNP) 分析、比较基因组杂交 (CGH) 分析
其他說明
法律資訊
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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分析證明 (COA)
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aCGH technology enhances chromosomal analysis, requiring significant DNA input per sample.
Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.
條款
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
GenomePlex® 擴增技術可提高土壤樣本的 DNA 產量,儘管可能會造成核酸酶損害,仍可確保下游分析的可靠性。
GenomePlex® Whole Genome Amplification 可有效地從動物樣本中萃取 DNA 進行基因組分析。
相關內容
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
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Is the WGA2 GenomePlex® Amplification Kit polymerase compatible with TA cloning?
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If starting with fragmented DNA, what is the smallest size fragment which can be successfully amplified with Product WGA2, GenomePlex® Amplification Kit, and do I still need to do the fragmentation step?
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The kit works best for fragments 400 bp and larger. When starting with fragmented DNA, we recommend: (1) skipping the fragmentation heat step, although the buffer should be added, and (2) increasing the PCR cycles from 14 to 20.
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Can I use Product WGA2, GenomePlex® Amplification Kit, to amplify DNA from a single cell?
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Using this kit to amplify DNA from a single cell is not recommended. We recommend using the GenomePlex Single Cell WGA Kit (WGA4) for such application. WGA4 was developed for use with single cells and includes an optimized cell lysis protocol which has been incorporated into the fragmentation step.
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What is the major difference between WGA1 and WGA2 kits?
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Functionally, WGA1 and WGA2 kits are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.
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Can I amplify single stranded DNA with WGA2, GenomePlex® Amplification Kit?
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When starting with single stranded starting materials, we recommend (1) skipping the fragmentation heat step, although the buffer should be added and (2) increasing the PCR cycles from 14 to 20. Note that if the ssDNA is actually cDNA from polyadenylated RNA, the kit will likely not give good representation of the input material, as the poly(T) ends constitute a large, non-random fraction.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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