The specific composition of the elution buffer E7777 is considered proprietary. However it is a Tris-HCL solution without EDTA.
推薦產品
一般說明
DNA结合到离心柱内的硅胶膜上。结合所得的DNA经过洗涤后,再在所选的缓冲液中洗脱所得的洁净、浓缩的DNA。每个离心柱至多可纯化100 μL或10 μg经PCR扩增的DNA,至多可纯化出 95%的100 bp至10 kb大小的PCR产物。该试剂盒可去除99%以上的引物和大多数的引物二聚体(<40 bp)。
纯化所得的DNA可用于酶促反应、传统或自动化测序、克隆和微阵列分析。
應用
特點和優勢
- 可回收95%长度介于100 bp至10 kb之间的PCR产物
- 可在8分钟内纯化出多达100 μL或10 μg 的PCR扩增产物DNA。
- 可去除99%以上的引物和其他成分
- 可在8分钟内纯化出多达100 μl或10 μg 的PCR扩增产物DNA。
- 可回收95%长度介于100 bp至10 kb之间的PCR产物
- 可去除99%以上的引物和其他成分
- 不需要去除有机萃取引入的矿物油
- 相对于其他市场领先竞争对手,可多获得40%的纯化制备物
其他說明
原則
法律資訊
訊號詞
Warning
危險分類
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Central nervous system
儲存類別代碼
8A - Combustible corrosive hazardous materials
閃點(°F)
Not applicable
閃點(°C)
Not applicable
從最近期的版本中選擇一個:
分析證明 (COA)
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GenElute™ PCR Clean-up Kit rapidly purifies PCR products from excess components, ensuring clean samples for downstream applications.
GenElute™ PCR Clean-up Kit 可快速純化 PCR 產品中的多餘成分,確保下游應用的樣品乾淨。
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
相關內容
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
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What is the elution buffer made of? As it can be replaced with water, is it just water that is provided?
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Helpful?
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Does this kit remove proteins from PCR product?
1 answer-
This is a post-pcr clean up kit. The single or double-stranded amplification products are bound to the silica membrane. Then all non-DNA material such as primers, nucleotides, and polymerase are washed away. Finally the purified product is eluted. Anything that isn't bound will be removed.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product No. NA1020, GenElute™ PCR Clean-Up Kit, be used to clean up PCR products larger than 10 kb?
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We haven't tested anything bigger than 10 kb, but we would expect larger DNA to bind well. That said, the bound DNA might be more difficult to elute. Heating the elution solution to 60-65°C may help with elution. Incubating a few minutes to allow the elution solution to soak into the binding matrix may also help.
Helpful?
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Can Product NA1020, GenElute™ PCR Clean-Up Kit, be used for isolation of PCR products labeled with digoxigenin?
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Although we have not tested the kit with digoxigenin-labeled PCR products specifically, it should be fine to use
Helpful?
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When using Product No. NA1020, GenElute™ PCR Clean-Up Kit, what is the largest amount of PCR reaction sample that can be processed on one column?
1 answer-
The upper limit on the PCR reaction volume that can be cleaned up is 100 μl. In that scenario, 500 μl of binding solution would be added for a total of 600 μl volume being applied to the column.
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When using GenElute™ PCR Clean-Up Kit, Product No. NA1020, Can the amount of elution buffer be reduced in order to obtain a more concentrated eluate?
1 answer-
One may indeed increase concentration by reducing elution volume, but we would not suggest going lower than 25-30 μl. One must keep in mind that if a volume of less than 50 μl is used (for example, 30 μl), the concentration will increase, but the total yield will also be reduced.
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Can GenElute™ PCR Clean-Up Kit, Product NA1020, be used for isolation of labeled PCR products?
1 answer-
This kit will work with labeled PCR products and no modifications to the standard protocol are needed.
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