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83330

Sigma-Aldrich

丙酮酸激酶 来源于兔肌肉

crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)

同義詞:

ATP:丙酮酸盐 2-O-磷酸转移酶, PK

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

形狀

suspension

品質

crystalline suspension in 3.8 M ammonium sulfate solution

比活性

~350 U/mg protein (10-40 mg/ml)

分子量

Mr ~230000

pH值

~6

異物活動

lactate dehydrogenase ≤0.01%

儲存溫度

2-8°C

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生化/生理作用

分子量:237 kDa,以分子量为 57 kDa 的四个等亚基的四聚体形式存在。
等电点:7.6
最佳 pH:~7.5
最佳温度:25°C
εA 280= 0.541 mg (p)/ml,1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中,报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性,但可利用其他三磷酸二核苷酸作为底物替代 ATP,包括 GTP、ITP、dATP、UTP 和 CTP。
Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.

單位定義

1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Patrícia Machado et al.
PloS one, 7(10), e47071-e47071 (2012-10-20)
Pyruvate kinase (PK) deficiency, causing hemolytic anemia, has been associated to malaria protection and its prevalence in sub-Saharan Africa is not known so far. This work shows the results of a study undertaken to determine PK deficiency occurrence in some
Jidong Cheng et al.
Genes to cells : devoted to molecular & cellular mechanisms, 17(11), 913-922 (2012-10-20)
AMP deaminase (AMPD) catalyzes AMP to IMP and plays an important role in energy charge and nucleotide metabolism. Human AMPD3 deficiency is a type of erythrocyte-specific enzyme deficiency found in individuals without clinical symptoms, although an increased level of ATP
Beverly A Teicher et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 18(20), 5537-5545 (2012-10-17)
The understanding that oncogenes can have profound effects on cellular metabolism and the discovery of mutations and alterations in several metabolism-related enzymes--isocitrate dehydrogenase 1 (IDH1), isocitrate dehydrogenase 2 (IDH2), succinate dehydrogenase (SDH), fumarate hydratase (FH), and pyruvate kinase M2 (PKM2)--has
Karl Kochanowski et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(3), 1130-1135 (2013-01-02)
Regulation of metabolic operation in response to extracellular cues is crucial for cells' survival. Next to the canonical nutrient sensors, which measure the concentration of nutrients, recently intracellular "metabolic flux" was proposed as a novel impetus for metabolic regulation. According
James M Urness et al.
Biochemistry, 52(1), 1-3 (2012-12-22)
A series of substrate analogues has been used to determine which chemical moieties of the substrate phosphoenolpyruvate (PEP) contribute to the allosteric inhibition of rabbit muscle pyruvate kinase by phenylalanine. Replacing the carboxyl group of the substrate with a methyl

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