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Merck
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重要文件

78948

Sigma-Aldrich

Atto 425叠氮化物

suitable for fluorescence

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About This Item

經驗公式(希爾表示法):
C30H43N5O8
分子量::
601.69
分類程式碼代碼:
12352200
PubChem物質ID:

化驗

≥80.0% (HPCE)

形狀

solid

製造商/商標名

ATTO-TEC GmbH

螢光

λex 436 nm; λem 484 nm±10 nm in PBS, pH 7.4

適合性

suitable for fluorescence

儲存溫度

−20°C

SMILES 字串

CC(CC(C)(C)N1CCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)C(C1=C2)=CC3=C2OC(C(C(OCC)=O)=C3)=O

InChI

1S/C30H43N5O8/c1-5-42-28(37)24-18-22-17-23-21(2)20-30(3,4)35(25(23)19-26(22)43-29(24)38)10-6-7-27(36)32-8-11-39-13-15-41-16-14-40-12-9-33-34-31/h17-19,21H,5-16,20H2,1-4H3,(H,32,36)

InChI 密鑰

RSOCGOAHHWBJFE-UHFFFAOYSA-N

一般說明

Atto 425是一种具有香豆素结构的荧光标记物。该染料预期用于生命科学领域,如标记DNA、RNA或蛋白质等。标记物的特征是吸收性强、荧光量子产量高、斯托克斯位移大、光稳定性良好和分子量低。
Atto 425具有中等亲水性。它的荧光可以在405-455 nm范围内有效激发。
叠氮化物修饰适用于与炔烃基团的反应(Huisgen反应 - “点击化学”)。

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法律資訊

本品仅供研究使用。如果打算商业化,请联系知识产权持有者(德国ATTO-TEC GmbH公司)申请许可。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Katrin G Heinze et al.
Biophysical journal, 86(1 Pt 1), 506-516 (2003-12-26)
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
Benzothiazole dye thioflavin T (ThT) is a sensitive probe for amyloid fibril detection. The ThT probing is based on its unique ability to form highly fluorescent complexes with amyloid and amyloid-like fibrils. In this work we propose an approach of
Triple FRET: a tool for studying long-range molecular interactions.
Elke Haustein et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 745-748 (2003-08-07)
Irina M Kuznetsova et al.
PloS one, 7(2), e30724-e30724 (2012-03-03)
A new approach for the determination of the amyloid fibril - thioflavin T (ThT) binding parameters (the number of binding modes, stoichiometry, and binding constants of each mode) is proposed. This approach is based on the absorption spectroscopy determination of
Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution.
Praus, P., et al.
Spectroscopy: An International Journal, 24, 303-307 (2010)

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