66540632

BIONi010-C-17

Human iPS Cell Line

• 分類程式碼代碼: 41106509

屬性

• 生物源: human
• 重編程方法: episomal
• 描述: age (N/A)
• 製造商／商標名: EBiSC^™
• 性別: male
• 增長模式: adherent (pluripotent)
• 技術: cell culture | stem cell: suitable
• 相關疾病: Alzheimer′s disease
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

一般說明

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

細胞系來源

Depositor
Bioneer

細胞系描述

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: Unknown

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Morphology:
TREM2 KO cl. 24-54 D1 thaw aft_ekU7aDe.tif - Phase contrast image 1 day after thawing
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 ecto D5 10x.tiff
Protocol or reference:
BIONi010-C-17 ecto D5.tif - Flow cytometry of in vitro differentiation to ectoderm
Endoderm:
Endoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 endo D5 10x.tiff
Protocol or reference:
BIONi010-C-17 endo D5.tif - Flow cytometry of in vitro differentiation to endoderm
Mesoderm:
Mesoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 meso D5 10x.tiff
Protocol or reference:
BIONi010-C-17 meso D5.tif - Flow cytometry of in vitro differentiation to mesoderm
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative

Genotyping
Karyotyping
Passage number: 35
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Image / Result

Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimer′s disease
Type of modification: Gene knock-out
Gene: TREM2
Chromosome location: KO of exon 1 of TREM2 Transcript variant 1 NM_018965
Delivery method: CRISPR-associated (CRISPR/Cas) System

聯結

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

例行更新培養

Surface coating: Matrigel^®/Geltrex^®
Passage method: Enzyme-free cell dissociation / EDTA
O₂ concentration: 20%
CO₂ concentration: 5%
Culture medium: Essential E8^®
Passage Number banked (pre-EBiSC): 35
No. Vials banked (pre-EBiSC): 30

法律資訊

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable