生物源
human dermis (fibroblast)
重編程方法
Sendai virus
描述
age (70-74)
製造商／商標名
EBiSC™
性別
male
增長模式
adherent (pluripotent)
技術
cell culture | stem cell: suitable
相關疾病
none
運輸包裝
dry ice
儲存溫度
−196°C
一般說明
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
細胞系來源
Depositor
Wellcome Trust Sanger Institute
Wellcome Trust Sanger Institute
細胞系描述
Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi022-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)POU5F1 (OCT-4)(+)SOX2(+)
PluriTest: Pluripotency score: 30.739
Novelty score: 1.417
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123000
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: WTSIi022-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)POU5F1 (OCT-4)(+)SOX2(+)
PluriTest: Pluripotency score: 30.739
Novelty score: 1.417
Microarray data: http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123000
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
聯結
Note: EAUA and CLIP must be completed before order fulfillment
例行更新培養
Medium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C
法律資訊
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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